WebMay 31, 2024 · For the cryo-SEM, the mycelial agar blocks of the FFPRI 460542 strain was attached to a specimen holder using the frozen embedding medium (Shandon Cryomatrix, Thermofisher Scientific, Tokyo, Japan), which was cryofixed by direct immersion in cooled hydrochlorofluorocarbon at –150 °C and transferred to the cold stage of a cryo-SEM … WebJun 30, 2024 · Following euthanasia, the eyes were dissected, and the eyecups were prepared and fixed in 4% paraformaldehyde (PFA, pH 7.4) for 20 min, cryoprotected, embedded in Shandon Cryomatrix, cut at 12 µm in a cryostat, mounted onto Super Frost Ultra Plus slides and stored at −80 °C until use.

Embedding Frozen Tissue Specimens - University of …

WebSecure and support tissue with Epredia™ Cryomatrix™ embedding resin, an outstanding product used to support tissue for frozen sectioning. Brand: Epredia™ 6769006. … WebJan 13, 2024 · For immunofluorescence staining, the tissues were embedded in Shandon Cryomatrix gel (Thermo Fisher Scientific), and 5 μm sections were prepared on Superfrost Ultra Plus slides by cryostat (Cryostar NX50). Preparations of formalin-fixed paraffin-embedded (FFPE) biopsy samples Tissues were fixed with 10% formalin for 24—48 h at … duthus

Shandon Cryomatrix Resin Thermo Fisher Bioz

WebProduct Name Shandon Cryomatrix Cat No. : 6769006 Synonyms No information available Recommended Use Laboratory chemicals. Uses advised against No Information available … Weba Shandon™ Cryomatrix™ (ThermoFisher Scienti c, Marietta, OH, USA) mounting medium. Sections with thicknesses of 10 mm were deposited on the silicon wafers (CrysTec GmbH, Ber-lin, Germany) for IR and MS imaging. The silicon substrates were surface modi ed with (3-aminopropyl)-triethoxysilane in WebSep 30, 2009 · Sludge granule samples were washed with deionized water and fixed with 4% paraformaldehyde. The fixed sludge granule samples were then dehydrated by successive passages through 50%, 80%, and 100% ethanol (10 min each) and were subsequently embedded in Shandon cryomatrix (Thermo Scientific, Pittsburg, PA, USA) and frozen at … duthsagar waterfall visiting time