WebWe have used kymograph analysis combined with edge detection and an automated computational algorithm to analyze the axonal transport kinetics of neurofilament polymers in cultured neurons at 30 ms temporal resolution. We generated 301 kymographs from 136 movies and analyzed 726 filaments ranging fr … WebChloramphenicol Optic Neuropathy Victor Godei, MD, PhD; Pinhas Nemet, MD; Moshe Lazar, MD • Three patients with cystic fibrosis of the pancreas initially were seen because of optic neuropathy caused by a toxic reaction to chloramphenicol. The ocular symptoms were characterized by loss of visual acuity, central scotomas, red-green dyschromatopsia, and …
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Kymograph - an overview ScienceDirect Topics
WebFine structure extraction of a kymograph using the rotational watershed method. Although traces extracted using the one-dimensional watershed algorithm (Figure ... using a total internal reflection fluorescence microscopy system (C1; Nikon) and movies were recorded using a CCD camera (Cascade 650; Photometrics Co.) as previously described 6, ... WebAug 12, 2016 · The method requires a compound microscope equipped with a GFP filter set and a sensitive camera, but does not require confocal microscopy, and is therefore a technique that can be used by most biology laboratories. ... In the “Kymograph” panel (Fig. 3), adjust “Line Width” to include the brightest part of cilium (~5–10 pixels). For ... WebKymograph is a device to record the magnitude of physiological variables, such as: muscle contraction. However, we observe some lacks of the conventional kymographs, such as: result’s... mobaxterm highlight keywords