Circrna pulldown
WebcircRNA过表达及干扰 miRNA过表达及干扰 lncRNA过表达及干扰 荧光素酶报告基因载体构建 Cre-LoxP系统工具载体设计与构建 CRISPR/Cas9基因编辑工具载体设计与构建 嗜神经病毒载体 狂犬病毒 水泡性口炎病毒 伪狂犬病毒 犬腺病毒 单纯疱疹病毒 溶瘤病毒载体 单纯疱疹 … WebMay 14, 2024 · RNA PULL DOWN技术了解一下. LncRNA是一类大于200nt的非编码RNA分子，在个体发育和疾病进程中都发挥了重要的作用。. 近年来，LncRNA的研究重点已经从早期的大规模鉴定，转移到了对具体的某个LncRNA的功能和机制的探索。. LncRNA主要通过与其他分子相互作用发挥功能 ...
Circrna pulldown
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WebJun 2, 2024 · A novel and highly specific circRNA-pulldown followed by mass spectrometry analysis identified 94 circFOXK2-interacting proteins, which were involved in cell adhesion, mRNA splicing, and structural molecule activity. Of these, circFOKX2 interactions with YBX1 and hnRNPK enhanced expression of oncogenes NUF2 and PDXK. WebAug 21, 2024 · The proteins associated with the circRNA can be studied using methods such as mass spectrometry and Western blotting, while the RNAs present in the …
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WebJun 1, 2024 · A novel and highly specific circRNA-pulldown followed by mass spectrometry analysis identified 94 circFOXK2-interacting proteins, which were involved in cell adhesion, mRNA splicing, and structural molecule activity. Of these, circFOKX2 interactions with YBX1 and hnRNPK enhanced expression of oncogenes NUF2 and PDXK. WebDec 1, 2024 · CircRNA-centric methods to study circRNA-protein interactions3.1. Antisense-based pulldown purification of circRNPs. The principle of antisense …
WebSep 22, 2024 · Researchers in the circRNA-RNA field can simply adapt to this pull-down method followed by the use of commercialized RNA extraction kits. In summary, this approach can be used to identify the …
WebJan 8, 2024 · CircRNA pulldown assay was carried out using Pierce TM Magnetic RNA-Protein Pull-Down Kit (No: 20164, Thermo Fisher Scientific, United States), all … titan tankless water heater prv requirementWebFeb 1, 2024 · Importantly, using circRNA pulldown assay and RNA-binding protein immunoprecipitation, we identified PABPC1 as a robust novel interacting protein of circPTK2. Mechanistically, circPTK2 could bind to PABPC1 and enhance its ability to stabilize SETDB1 mRNA, thereby specifically promoting SETDB1 expression and … titan tankless water heater not heatingWebJul 9, 2024 · IntegrateRNA provides a one-stop solution to accelerate the discovery and preclinical development of circRNA biomarkers. RNA pull-down is an in vitro technique … titan tankless water heater warrantyWebFeb 15, 2024 · Identification of the circRNA-binding proteins can be achieved by circRNA-pulldown method [33,34,35]. Molecular mechanisms and functions of circular RNA … titan tankless water heater replacementWebCircRNA pull-down assay. A biotin-labeled circPTPRA probe was designed and synthesized by RiboBio Co., Ltd. The RNA pull-down assay was performed according to the instruction of the Pierce™ Magnetic RNA–Protein Pull-Down Kit (Thermo Scientific). The probe sequences used for the RNA pull-down assay are summarized in Additional … titan tarp systems incWebJan 4, 2024 · The oncogenic roles of circRTN4 in PDAC tumor growth and metastasis were studied in mouse tumor models. Bioinformatics analysis, luciferase assay and miRNA pulldown assay were performed to study the novel circRTN4-miRNA-lncRNA pathway. To identify circRTN4-interacting proteins, we performed circRNA-pulldown and mass … titan tankless water heater reed valveWebcircRNA Pull-down Assay IntegrateRNA is an outstanding biotechnology company with years of experience in RNA-protein interaction analysis. We offer customized circRNA … titan tankless water heater repair